Fig. 3
From: Description of CRISPR/Cas9 development and its prospect in hepatocellular carcinoma treatment
The comparison between typical sgRNA mediated CRISPR/Cas9 and Cas9 nickase. a Representative schematic of sgRNA mediated CRISPR/Cas9. The sgRNA derives from tracrRNA-crRNA complex. Each strand is cleaved by a distinct Cas9 nuclease domain (HNH or RuvC). b, c Representative schematic of Cas9 nickase. One of Cas9 domains inactivation results in single strand cleavage and HDR repairment instead of double strands break. Abbreviations: sgRNA, single strand RNA; PAMs, protospacer adjacent motifs