Fig. 2

a Western blot analysis and relative quantification by densitometric analysis of total or acetylated histone H3 pan (Ac-H3) or at K9 (Ac-H3K9), K27 (Ac-H3K27), K56 (Ac-H3K56), and histone H4 at K16 (Ac-H4K16) and α-tubulin at K40 (Ac-tubulin) in extract of HUVEC exposed to indicated concentrations of CPTH6 for 72 h. Values are expressed as fold change of acetylated proteins relative to total ones. b Immunofluorescence images (left) and relative quantification (right) of tyrosinated (Tyr) and acetylated (Ac) α-tubulin in HUVEC exposed to increasing concentrations of CPTH6 for 72 h. p-values were calculated between control and treated cells. *p < 0.05. Magnification 63X. Scale bar, 50μm