Fig. 1
CCDC50 exon 6 skipping event is significant in ccRCC and is correlated to poor prognosis. a Flow chart of the screening process of CCDC50 alternative splicing event. The data of 72 pairs of ccRCC and adjacent noncancerous tissues in TCGA were analyzed. The percent spliced-in (PSI) value were calculated and candidate exon skipping events were identified when the absolute difference value between the cancer group and normal one was higher than 0.3 with statistical significance. b The PSI value of CCDC50 exon 6 in 72 pairs of ccRCC and adjacent noncancerous tissues was calculated. c The PSI values of CCDC50 exon 6 in 33 types of cancer and normal tissues were acquired from TCGA SpliceSeq Database. Significantly lower mean PSI in tumor tissues was showed in KIRC. d The relative expression level of CCDC50 exons in ccRCC and normal tissues. e Kaplan-Meier curve showing (a) recurrence free survival and (b) overall survival of renal cancer patients in TCGA with high or low PSI values of CCDC50. (Pa = 0.0212, Pb = 0.5753 by log-rank test). f Kaplan-Meier curve showing (a) recurrence free survival and (b) overall survival of 48 ccRCC patients from Tongji Hospital with high or low PSI values of CCDC50. (Pa = 0.0240, Pb = 0.0469 by log-rank test)