Fig. 6

ZNF395 is a downstream oncogene of CCDC50-V1. a The expression of ZNF395 was determined in 786-O and OS-RC-2 cells by (a) RT-qPCR and (b) western blot after the CCDC50 knockdown or CCDC50-S overexpression. GAPDH and β-actin were used as the internal control of RT-qPCR and western blot respectively. b The expression of ZNF395 was examined in (a) normal HK2 cell line and four renal cancer cell lines (ACHN, OS-RC-2, 786-O, A498), (b) ccRCC and corresponding normal tissues by RT-qPCR. GAPDH was used as the internal control. c Colony formation assays were utilized to examine the proliferative ability of 786-O and OS-RC-2 cells with ZNF395 knockdown. d EdU incorporation assays were conducted in ZNF395 silenced 786-O and OS-RC-2 cells. e-f Migration and invasion assay were performed in 786-O and OS-RC-2 cells expressing sh-ZNF395. g Western blot was implemented to identify downstream genes and pathways of ZNF395 in 786-O and OS-RC-2 cells. β-actin was used as the internal control