Fig. 5
ACYP2 promotes STAT3 phosphorylation in glioma cells through regulating intracellular Ca2+ homeostasis and calpain activity. Cells transfected with the indicated shRNAs were treated with 5 μM BAPTA-AM for 6 h (a), 10 μM calpeptin for 10 h (b), 10 μM Na3VO4 for 1 h (c), or the vehicle. Western blot analysis was then performed to test their effect on STAT3 phosphorylation. GAPDH was used as a loading control. d, Cells transfected with the indicated shRNAs were treated with the vehicle or 10 μM Na3VO4 for 1 h, and the MTT assay was then carried out to evaluate their effect on cell proliferation. The data were presented as mean ± SD (n = 3). Cells stably knocking down ACYP2 and control cells were transfected with siRNA targeting PTP1B or not. e, Western blot analysis was then performed to evaluate their effect on STAT3 phosphorylation. f, MTT assay was performed to assess their effect on cell proliferation. The data were presented as mean ± SD (n = 3). **, P < 0.01; ***, P < 0.001