Fig. 1
From: 2,6-DMBQ is a novel mTOR inhibitor that reduces gastric cancer growth in vitro and in vivo
2,6-DMBQ reduces growth of gastric cancer cells. a Chemical structure of 2,6-DMBQ. b Effect of 2,6-DMBQ on growth of gastric cancer cells. Cells were treated with 2,6-DMBQ at various concentrations and then incubated for 48 h and growth was determined by the MTT assay. c Effect of 2,6-DMBQ on anchorage-independent growth of gastric cancer cells. Cells were treated with 2,6-DMBQ and incubated for 2 weeks and then colonies were counted using a microscope and the Image-Pro PLUS (v.6) computer software program. d Effect of 2,6-DMBQ on cell cycle. HGC27 gastric cancer cells were synchronized by serum starvation for 24 h and treated with 2,6-DMBQ for 24 h in 10% serum-supplemented medium. Cells were stained with propidium iodide (PI) and cell cycle was analyzed by Fluorescence Activated Cell Sorting (FACS). For b-d, data are shown as means ± S.D. of values from 3 independent experiments each with triplicate samples and the asterisk (*) indicates a significant (p < 0.05) difference. e Effect of 2,6-DMBQ on the expression of cell cycle marker proteins in HGC27 gastric cancer cells was determined by Western blotting. Band density was measured using the Image J (NIH) software program. For e, similar results were observed from 3 independent experiments