Fig. 2
From: 2,6-DMBQ is a novel mTOR inhibitor that reduces gastric cancer growth in vitro and in vivo
2,6-DMBQ induces apoptosis of gastric cancer cells. a Effect of 2,6-DMBQ on cancer cell death. Cells were seeded in a 6-well plate and treated with 2,6-DMBQ for 48 h. The number of suspended or attached cells was counted using a hematocytometer. b Effect of 2,6-DMBQ on cancer cell apoptosis. Cells were treated with 2,6-DMBQ in 10% FBS and then incubated for 48 h. Cells were stained with annexin V and propidium iodide (PI) and apoptosis was determined by Fluorescence Activated Cell Sorting (FACS). For a-b, data are shown as means ± S.D. of triplicate values from 3 independent experiments and the asterisk (*) indicates a significant (p < 0.05) difference. c Effect of 2,6-DMBQ on expression of proapoptotic marker protein. Cells were treated with 2,6-DMBQ for 48 h and the levels of cleaved PARP protein was determined by Western blotting. Band density was measured using the Image J (NIH) software program. For c, results of Western blotting are shown as mean values ± S.D. for 3 independent experiments