Fig. 5
From: 2,6-DMBQ is a novel mTOR inhibitor that reduces gastric cancer growth in vitro and in vivo
Reduction of cell growth by 2,6-DMBQ is dependent on the expression of mTOR. a The effect of 2,6-DMBQ on gastric cancer cell growth was assessed in cells stably expressing shmTOR or cells stably expressing shControl. Cells were seeded for 24 h and treated or not treated with 2,6-DMBQ at various concentrations and then incubated for 48 h and growth was determined by the MTT assay. b The effect of 2,6-DMBQ on anchorage-independent gastric cancer cell growth was assessed in cells stably expressing shmTOR or cells stably expressing shControl. Cells were treated with scutellarin and incubated for 2 weeks and then colonies were counted using a microscope and the Image-Pro PLUS (v.6) computer software program. All data are represented as means ± S.D. of triplicate values from 3 independent experiments. The asterisk (*) indicates a significant (p < 0.05) difference