Fig. 6
From: 2,6-DMBQ is a novel mTOR inhibitor that reduces gastric cancer growth in vitro and in vivo
2,6-DMBQ reduces gastric cancer patient-derived xenograft tumor growth in vivo. Mice were divided into 2 groups to assess the effect of 2,6-DMBQ on gastric cancer patient-derived xenograft tumor growth. Groups are as follows: 1) vehicle group or 2) group treated with 80 mg/kg of 2,6-DMBQ. Tumor-bearing mice were orally administered (by gavage) 2,6-DMBQ or vehicle once a day Monday through Friday for 43 days. Tumor volumes were measured on the days indicated. The effect of 2,6-DMBQ on gastric tumor growth in (a) LSG55 or (b) LSG64 gastric PDX tissues. The asterisk (*) indicates a significant difference between tumors from untreated or treated mice as determined by t test (p < 0.05). c Effect of 2,6-DMBQ on Ki-67 expression. Treated or untreated groups of tumor tissues were stained with antibodies to detect Ki-67. d Effect of 2,6-DMBQ on the mTOR signaling pathway. Treated or untreated groups of tumor tissues were stained with antibodies to detect phosphorylated mTOR and phosphorylated p70S6K. For c and d, the number of Ki-67, phosphorylated mTOR or phosphorylated p70S6K-stained cells was counted from immunohistochemistry results (n = 6). All data are shown as means ± S.E. of values obtained from the experiment groups. The asterisk (*) indicates a significant difference between tumors from untreated or treated mice as determined by t test (p < 0.05)