Fig. 5From: VHL mutation-mediated SALL4 overexpression promotes tumorigenesis and vascularization of clear cell renal cell carcinoma via Akt/GSK-3β signalingSALL4 downregulation attenuates the activation of Akt/GSK-3β signaling and VEGFA expression in ccRCC. a, b Western blot analyses for protein levels of total Akt (t-Akt), phosphorylated Akt (p-Akt), total GSK-3β (t-GSK-3β), phosphorylated GSK-3β (p-GSK-3β) and VEGFA in ACHN (a) and 786-O (b) cells with indicated treatment. c Immunofluorescence staining of p-Akt and p-GSK-3β in ACHN (left) and 786-O (right) cells with indicated treatment (scale bar, 50 μm)Back to article page