Fig. 5

miR-424 decreases the sensitivity of bladder cancer cells to CDDP in vivo. Five thousand six hundred thirty-seven cells transduced with the empty lentiviral vector or with the lentiviral vector for miR-424 were inoculated into nude mice. Fourteen days after inoculation, mice were treated with CDDP or with vehicle alone. At the indicated time points after inoculation, volumes of the tumors were measured (a). The representative pictures of the surgically removed tumors of each group were taken (b). c Western blot. Cell lysates prepared from the indicated xenograft tumor tissues were subjected to Western blot with the indicated antibodies. β-actin was used as a loading control. d-f Immunohistochemical staining. Paraffin-embedded sections prepared from the indicated xenograft tumor tissues were analyzed for UNC5B (D), SIRT4 (e) and PARP (f) by immunohistochemical staining. (G-H) miR-424 expression increases and decreases number of Ki67-positive and TUNEL-positive cells, respectively. The formaldehyde-fixed paraffin-embedded xenograft tumors were cut into 4-μm-thick sections and stained with anti-Ki67 antibody (g) or with TUNEL (h). % Ki67- or TUNEL-positive cells were shown (right panels). The data are presented as the mean ± S.E. *P < 0.05, **P < 0.01. Scale bars, 25 μm