Fig. 8From: 3,3′-Diindolylmethane modulates aryl hydrocarbon receptor of esophageal squamous cell carcinoma to reverse epithelial-mesenchymal transition through repressing RhoA/ROCK1-mediated COX2/PGE2 pathwayIn vivo assay and co-IP assay verifies that DIM can modulate AHR to reverse EMT through repressing RhoA/ROCK1-mediated COX2/PGE2 pathway. a. IHC results of xenografts staining showed downexpression of AHR, RhoA, ROCK1, COX2 and mesenchymal cell markers as well as upregulation of epithelial cell marker Claudin-1. b. Co-IP assay indicated that proteins had direct interactions between each other. DIM+: 40 μM. c. Overexpression of AHR resulted in elevated levels of p-EGFR. d. DIM (40 μM) could inhibit expressions of EGFR and p-EGFR as well as NF-κB p65 and p-p65. e. Schematic representation of molecular mechanism of DIM in reversal of EMT process of ESCCBack to article page