Fig. 3

Overexpression of H19 promotes EMT in colorectal cancer cells. a Correlation analysis of H19 and Snail expression in TCGA database. b GSEA of EMT gene signatures in colorectal cancer samples with high H19 expression versus those with low H19 expression in TCGA database. The median expression of H19 was used as cut-off. c Effect of forced expression of H19 on the mRNA level of Snail in HCT116 and SW480 cells were measured by qRT-PCR. d Effect of H19 knockdown on the mRNA level of Snail in HCT116 or DLD1 H19 cells were quantified by qRT-PCR. e Western blot analysis of EMT markers by ectopic expression of H19 in HCT116 and SW480 cells. f Western blot analysis of EMT markers in H19 knockdown or control cells. Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001