Skip to main content
Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Long non-coding RNA H19 promotes colorectal cancer metastasis via binding to hnRNPA2B1

Fig. 6

H19 stabilize RAF1 by interacting with hnRNPA2B1. a The effect of hnRNPA2B1 knockdown on the mRNA level of Raf kinase family was measured by qRT-PCR. b The influence of H19 overexpression on the mRNA level of Raf kinase family were measured by qRT-PCR in colorectal cancer cells. c The impact of H19 knockdown on the mRNA level of Raf kinase family were measured by qRT-PCR in colorectal cancer cells. d Western blot analysis of Raf-1 in H19 stable overexpression or control cells. e Western blot analysis of Raf-1 after hnRNPA2B1 knockdown in HCT116 and SW480 cells. f Correlation analysis of H19 and Raf-1 in primary colorectal cancer tissues. g Overexpression of Raf-1 rescued the migration and invasion of H19-depleted HCT116 cells. h Western blot analyses of EMT markers after overexpression of Raf-1 in H19-depleted HCT116 cells. i RIP assay followed by qRT-PCR explored the enrichment of Raf-1 mRNA binding to hnRNPAA2B1. j RIP experiment showed that H19 overexpression increased the enrichment between Raf-1 mRNA and hnrnpA2B1. k Knockdown of hnRNPA2B1 disrupted the stability of Raf-1 mRNA compared with the control group. l Overexpression of H19 increased the stability of Raf-1 mRNA compared with the control group. m Knockdown of hnRNPA2B1 reversed H19-induced Raf-1 mRNA stabilization. Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page