Fig. 2

Effect of BDH2 on cell growth. a, b BDH2 was silenced or downregulated in GC cell lines, but had steady expression in normal gastric tissue as determined by qRT-PCR and western blot analyses. c SGC7901 and BGC823 cells were transfected with an empty vector or BDH2 expression vector to establish stably BDH2-overexpressing cell lines. Exogenous expression of BDH2 was detected by western blotting. d CCK-8 assays showed that BDH2 significantly inhibited cell viability. e Colony formation abilities of SGC7901 and BGC823 cells were markedly hampered by BDH2 overexpression. f Flow cytometry was performed to detect the effects of BDH2 on the cell cycle. g Flow cytometry was used to evaluate the effect of BDH2 on apoptosis. Apoptotic cells were detected by ANXA5 and PI staining. h Expression levels of apoptosis markers in BDH2-expressing cells were confirmed by western blot analysis. Results are presented as means ± S.D. (n = 3); *p < 0.05, **p < 0.01