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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: BDH2 triggers ROS-induced cell death and autophagy by promoting Nrf2 ubiquitination in gastric cancer

Fig. 5

ROS induced by BDH2 overexpression promotes apoptosis and autophagy in GC cells. a–i BDH2-overexpressing cells with or without NAC treatment. a BDH2-overexpressing and vector control cells were stained with DCFH-DA to measure intracellular ROS production by flow cytometry. b Quantitative analysis of ROS generation. c Cell viability was measured by CCK-8 assays. d Quantitative analysis of the percentage of apoptotic cells. e–h Detection of autophagic flux by the GFP-mRFP-LC3 lentivirus in BDH2-overexpressing cells. Scale bars, 20 μm. i Protein expression levels of LC3-II, p62, cleaved caspase-3, and cleaved-PARP were detected by western blotting. j Frozen tissue sections were stained with the DCFH-DA probe and tissue ROS levels were observed by fluorescence microscopy. Scale bars, 20 μm. Results are presented as means ± S.D. (n = 3); *p < 0.05, **p < 0.01 compared with vector; ##p < 0.01 compared with BDH2 transfected cells treated with NAC

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