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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Mitochondrial ROS accumulation inhibiting JAK2/STAT3 pathway is a critical modulator of CYT997-induced autophagy and apoptosis in gastric cancer

Fig. 4

CYT997 induced apoptosis through triggering intracellular mitochondrial ROS generation in SGC-7901 and MKN45 cells. a The level of ROS was detected in SGC-7901 and MKN45 cells treated with CYT997 by fluorescence microscopy. Scale bars = 50 μm. b ROS levels of SGC-7901 and MKN45 cells treated with CYT997 were determined by flow cytometry. The fold change in DCFH-DA intensity was shown in the right of bar. **p < 0.01. c Mitochondrial ROS stained with MitoSOX Red dye were detected by confocal microscope and flow cytometry. The fold change in MitoSOX Red intensity was shown in the right bar. Scale bars = 7.5 μm. d-e The mitochondrial membrane potential stained with the fluorescent mitochondrial probe JC-1 was measured and assessed by confocal microscope d and flow cytometry e. Scale bars = 5 μm.f SGC-7901 cells were treated with CYT997 alone or in combination with NAC (5 mM). Cell viability was detected by a CCK8 assay. **p < 0.01.g The cell cycle distribution of SGC-7910 cells treated with CYT997 alone or in combination with NAC (5 mM) was analyzed by flow cytometry. **p < 0.01. h SGC-7901 cells were treated with CYT997 alone or in combination with NAC (5 mM). Apoptosis was detected by flow cytometry. ** p < 0.01. i Expression of cell cycle, autophagy and apoptosis related proteins was detected by western blotting in SGC-7901 cells

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