Fig. 3

Sirt6 inhibition promoted cell apoptosis and cell cycle arrest in DLBCL cells. a Heatmaps of the Sirt6 correlated gene-expression signature in RNA-seq analysis. Columns represent samples and rows represent genes. b, c Functional enrichment analyses of differentially expressed genes according to RNA-seq of LY1 cells with Sirt6 knocked down, where b depicts the biological process of gene enrichment in the down-regulated group and c describes the biological process of up-regulated gene enrichment. In the GO Chord plot in b, the left side shows some significantly down-regulated genes (the blue color bar next to the gene represents down-regulation, and in c the red bar represents up-regulation), the right side shows different GO terms, and the colored ribbon connected to genes represent the genes enrichment in this GO term. The GO Chord plot on the left and the GO Enrichment score plot on the right in b and c represent the same meaning. d Sirt6 knockdown induced increased apoptosis rates in LY1, LY8, and Val cells as assessed by flow cytometric analysis with Annexin V-PE/7AAD staining. e Sirt6-depletion induced cell cycle arrest at the G2/M phase in LY1 and LY8 cells. Cell cycle distribution was detected using flow cytometry (mean ± SD, n = 3, **p < 0.01, ***p < 0.001). f) Decreased expression levels of CDK2 and increased levels of activated PARP and p27 were observed in sh-Sirt6-treated DLBCL cells