Fig. 3

Circ-ATXN1 was involved in SRSF10-mediated angiogenesis of GECs. a Relative enrichment of circ-ATXN1 in anti-IgG and anti-SRSF10 were detected by RNA immunoprecipitation assay. Data represent mean ± SD (n = 3, each group; **P < 0.01, ^##P < 0.01). b Effect of SRSF10 knockdown on expression of circ-ATXN1 was detected by qRT-PCR. Data represent mean ± SD (n = 3, each group; ** P < 0.01). c Effects of circ-ATXN1 and SRSF10 on the cell viability of GECs were detected by CCK-8 assay. Data represent mean ± SD (n = 3, each group; **P < 0.01, ^##P < 0.01, ^&&P < 0.01). d Effects of circ-ATXN1 and SRSF10 on the migration of GECs were detected by Transwell assay. Data represent mean ± SD (n = 3, each group; **P < 0.01, ^##P < 0.01, ^&&P < 0.01). e Effects of circ-ATXN1 and SRSF10 on tube formation of GECs were measured by Matrigel tube formation assay. (Black arrow, tube structures; Grey arrow, tube branches). Data represent mean ± SD (n = 3, each group; **P < 0.01, ^##P < 0.01, ^&&P < 0.01). Scale bar represents 30 μm. f-g Effects of circ-ATXN1 and SRSF10 on expressions of MMP2 and VEGFA were detected by western blot. Data represent mean ± SD (n = 3, each group; **P < 0.01, ^##P < 0.01, ^&&P < 0.01). h Effects of circ-ATXN1 and SRSF10 on activity of MMP2 were detected by gelatin zymography. Data represent mean ± SD (n = 3, each group; *P < 0.05)