Table 2 Primer and probes used for RT-qPCR
Primer or Probe | Gene | Sequence(5’->3’) or Assay ID |
|---|---|---|
Primer | SRSF10 | F:CCAGGGGGATCGAAAGACAC |
R:GTGGTCTTCCAGTCGGTCTAC | ||
Circ-ATXN1 | F:GTGCTTGCAGGTTTTCTAGGTAG | |
R:GTGGGTACAATCCGCCAAC | ||
P:FAM+AGCTCTGGATGTGCAATTGGCACTG+BHQ1 | ||
GAPDH | F:GGACCTGACCTGCCGTCTAG | |
R:TAGCCCAGGATGCCCTTGAG | ||
P:FAM+CCTCCGACGCCTGCTTCACCACCT+Eclipse | ||
Probe | miR-526-3p | 002383 (Applied biosystems) |
U6 | 001973 (Applied biosystems) |