Fig. 3

miR-130b-3p induces GC tumor formation and angiogenesis in vivo.a RT-qPCR was used to detect the expression of miR-130b-3p in tumors after injection of NUGC-3 and HGC27 cells. b Tumor volume changes in each group of nude mice. c Tumor weight of each group in the nude mice. d, e Western blot assay was used to detect the expression of each protein in the tumor normalized to GAPDH after injection of NUGC-3 and HGC27 cells. f Immunohistochemistry was used to detect CD31 expression in tumors of nude mice (200 ×). p < 0.05 vs. agomir-NC-injected nude mice; # p < 0.05 vs. antagomir-NC-injected nude mice. One-way ANOVA was used for statistical analysis among multiple groups, and repeated measure ANOVA was performed at different time points. n = 5 for each mouse group