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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: RETRACTED ARTICLE: M2 macrophage-derived extracellular vesicles promote gastric cancer progression via a microRNA-130b-3p/MLL3/GRHL2 signaling cascade

Fig. 4

M2 macrophage-derived EVs mediate miR-130b-3p to promote survival, migration, invasion and angiogenesis of GC cells. a The expression of M1 and M2 macrophage marker genes was detected by RT-qPCR. b RT-qPCR was used to detect miR-130b-3p expression in M2 macrophages. c The morphology of M2 macrophage-derived EVs was observed by a transmission electron microscope (scale bar = 100 nm). d Nanoparticle tracking analysis of EVs size distribution. e Western blot analysis for the expression of EV marker proteins TSG101, CD63 and CD81 normalized to GAPDH. f Fluorescence staining of PKH-67-labeled M2 macrophage-derived EVs were captured by NUGC-3 and HGC27 cells and HUEVCs (400 ×). g The expression of miR-130b-3p in M2 macrophages was detected by RT-qPCR. h The CCK-8 assay was adopted to detect the viability of cells. i The TUNEL assay was used to detect apoptosis of cells (200 ×). j Transwell assay was utilized to detect the migration of cells (200 ×). k Transwell assay was conducted to detect the invasion of cells (200 ×). l Matrigel-based angiogenic assays were performed to detect numbers of vessel-like tubes formed in vitro (100 ×). m, n Western blot assay was used to detect the expression of proteins normalized to GAPDH in each group. * p < 0.05 vs. untreated EV group; # p < 0.05 vs. M2-EV + NC group. Unpaired t test was used for the comparison between the two groups, and two-way ANOVA was performed at different time points

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