Fig. 5

miR-130b-3p targets MLL3 to promote GC cell proliferation, migration, invasion, and vessel-like tube formation of HUEVCs. a Venn plots of miR-130b’s downstream target gene results predicted by TargetScan (http://www.targetscan.org/vert_71/), miRWalk (http://mirwalk.umm.uni-heidelberg.de/), DIANA TOOLS (http://diana.imis.athena-innovation.gr/DianaTools), StarBase, miDIP (http://ophid.utoronto.ca/mirDIP/) and miRDB (http://www.mirdb.org/). b The PPI network diagram obtained in the String. The connection between genes indicates a relationship between genes. The color indicates the core degree. The red means the higher core degree, and the blue means lower core degree. c Dual luciferase reporter gene assay to detect the binding site between miR-130b-3p and MLL3. * p < 0.05 the mimic-NC group. d RT-qPCR was used to detect the expression of miR-130b-3p in GC tissues and adjacent normal tissues. * p < 0.05 vs. adjacent normal tissues. e Correlation analysis between miR-130b-3p and MLL3 by Pearson’s correlation coefficient. f MLL3 expression in NUGC-3 and HGC27 cells was detected by RT-qPCR. * p < 0.05 vs. agomir-NC-transfected cells; # p < 0.05 vs. antagomir-NC-transfected cells; & p < 0.05 vs. oe-NC-transfected cells; @ p < 0.05 vs. miR-130b-3p agomir + oe-NC-transfected cells. g CCK-8 assay was adopted to detect the viability of cells. h TUNEL assay was used to detect apoptosis of cells (200 ×). i Transwell assay was utilized to detect the migration of cells (200 ×). j Transwell assay was conducted to detect the invasion of cells (200 ×). k Matrigel-based angiogenic assays were performed to detect numbers of vessel-like tubes formed in vitro (100 ×). l, m Western blot assay was used to detect the expression of proteins normalized to GAPDH in each group. g-m * p < 0.05 vs. oe-NC-transfected cells; # p < 0.05 vs. miR-130b-3p agomir + oe-NC-transfected cells. Unpaired t test was used for the comparison between the two groups, and two-way ANOVA and Bonferroni test were performed for the comparison among multiple groups at different time points