Fig. 7

Overexpression of GRHL2 in GC cells alleviates the effects of miR-130b-3p from M2 macrophage-derived EVs. a RT-qPCR detection of miR-130b-3p expression in M2 macrophage-derived EVs. * p < 0.05 vs. cells with M2-EV + oe-NC treatment. b RT-qPCR detection of miR-130b-3p and GRHL2 expression in NUGC-3 and HGC27 cells treated with EVs. c CCK-8 assay was adopted to detect the viability of cells. d TUNEL assay was used to detect apoptosis of cells (200 ×). e Transwell assay was utilized to detect the migration of cells (200 ×). f Transwell assay was conducted to detect the invasion of cells (200 ×). g Matrigel-based angiogenic assays were performed to detect numbers of vessel-like tubes formed in vitro (100 ×). h, i Western blot assay was used to detect the expression of proteins normalized to GAPDH in each group. b-i * p < 0.05 vs. cells with M2-EV + oe-NC treatment; # p < 0.05 vs. cells with M2-EV + oe-miR-130b-3p treatment. One-way ANOVA and Tukey’s test were used for the comparison among multiple groups, and two-way ANOVA and Bonferroni test were performed at different time points