Fig. 8

Knockdown of miR-130b-3p in M2 macrophage-derived EVs or overexpression of GRHL2 inhibits GC tumor growth and angiogenesis in vivo. a RT-qPCR detection of miR-130b-3p and GRHL2 expression in tumors after injection of NUGC-3 and HGC27 cells. b Tumor volume change in each group. c Tumor weight change in each group. d, e Western blot analysis to detect the expression of each protein in tumor tissues normalized to GAPDH after injection of NUGC-3 and HGC27 cells. f CD31 expression in each group of tumors (200 ×). * p < 0.05 vs. mice without treatment; # p < 0.05 vs. mice injected with M2-EV-NC; & p < 0.05 vs. mice injected with oe-NC. One-way ANOVA and Tukey’s test were used for the comparison among multiple groups, and two-way ANOVA and Bonferroni test were performed at different time points. n = 5 for each mouse group