Fig. 7

RuCUR compound treatment in p53 null cells. (a) Western blot analysis of the indicated protein levels was performed in HCT116 p53−/−cells pre-treated with brusatol (100 nM for 4 h) prior to adding RuCUR (100 μM) for 24 h. Actin was used as protein loading control. The ratio of the protein levels vs β-actin, following densitometric analysis using ImageJ software, is reported. Ctr: positive control for p53. (b) Cell viability was measured by trypan blue exclusion assay in HCT116 p53−/−cells pre-treated with brusatol (100 nM for 4 h) prior to adding RuCUR (100 μM) for 24 h and expressed as cell death percentage ± S.D. * (p ≤ 0.01) (single treatments compared to untreated cells)