Fig. 6From: SM22α+ vascular mural cells are essential for vessel stability in tumors and undergo phenotype transition regulated by Notch signalingNotch activation alleviates the secretory phenotype in vSMCs. a RNA-seq data of AdCtrl- or AdNIC-transduced vSMCs-DA were analyzed for the expression of secretory phenotype-related genes and shown as a heatmap. b The expression of secretory phenotype-associated genes was analyzed by GSEA between the AdNIC and AdCtrl groups. c vSMCs-DA were transduced with AdCtrl or AdNIC for 48 h. The expression of secretory phenotype-associated genes was determined by qRT-PCR (n = 5). d vSMCs-DA were treated as in (c). The concentration of TNFα and Cxcl10 in the supernatant was determined using ELISA (n = 5). e Expression of TLR1, TLR2, TLR3 and TLR4 was evaluated using qRT-PCR (n = 5). f LLC and B16-F10 cells were plated in matrigel-coated Transwell chambers and cultured with CM derived from vSMCs-DA pre-transduced with AdNIC or AdCtrl. The invasion of LLC and B16-F10 cells was determined 24 h later by crystal violet staining (n = 5). g LLC cells were cultured with CM derived from vSMCs-DA pre-transduced with AdNIC or AdCtrl. The proliferation was evaluated 24 h later using the EdU incorporation assay (n = 5). Bars = means ± SD. *, P < 0.05; **, P < 0.01; and ***, P7 < 0.001Back to article page