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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Human umbilical cord mesenchymal stem cells-derived exosomes deliver microRNA-375 to downregulate ENAH and thus retard esophageal squamous cell carcinoma progression

Fig. 2

miR-375 suppressed ESCC cell proliferation, invasion, migration, and stemness while inducing apoptosis in vitro. A, The expression of miR-375 was determined by RT-qPCR in KYSE70 cells transfected with miR-375 mimic or inhibitor, relative to U6. B, Proliferation of KYSE70 cells in response to miR-375 mimic or inhibitor transfection evaluated by EdU staining (scale bar = 50 μm). C, Invasion and migration of KYSE70 cells in response to miR-375 mimic or inhibitor transfection evaluated by Transwell assay (scale bar = 50 μm). D, Tumorsphere formation of KYSE70 cells in response to miR-375 mimic or inhibitor transfection evaluated by tumorsphere formation assay (scale bar = 100 μm). E, Apoptosis of KYSE70 cells in response to miR-375 mimic or inhibitor transfection evaluated by flow cytometry. F, mRNA expression of Bcl-2, Bcl-xl, Bax, CD133, Nanog and OCT-4 was measured by RT-qPCR in KYSE70 cells, relative to GAPDH. G, Representative western blots of E-cadherin, N-cadherin, Snail, Bcl-2, Bcl-xl, Bax, CD133, Nanog and OCT-4 proteins and their quantitation in KYSE70 cells, relative to GAPDH. * p < 0.05 vs KYSE70 cells transfected with NC-mimic, # p < 0.05 vs KYSE70 cells transfected with NC-inhibitor by one-way ANOVA. Data are shown as mean ± standard deviation of three technical replicates

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