Fig. 6

miR-375 inhibited proliferation, migration, invasion and stemness, and enhanced apoptosis of KYSE70 cells via delivery of hUCMSCs-exo in vitro. A, Proliferation (A) of KYSE70 cells co-cultured with exo-miR-375 mimic or exo-miR-375 inhibitor evaluated by EdU staining (scale bar = 25 μm). B, Invasion and migration of KYSE70 cells co-cultured with exo-miR-375 mimic or exo-miR-375 inhibitor evaluated by Transwell assay (scale bar = 50 μm). C, Tumorsphere formation of KYSE70 cells co-cultured with exo-miR-375 mimic or exo-miR-375 inhibitor evaluated by tumorsphere formation assay (scale bar = 100 μm). D, Apoptosis of KYSE70 cells co-cultured with exo-miR-375 mimic or exo-miR-375 inhibitor evaluated by flow cytometry. E, The miR-375 expression and mRNA expression of ENAH, Bcl-2, Bcl-xl, Bax, CD133, Nanog and OCT-4 was measured using RT-qPCR in KYSE70 cells, relative to U6 and GAPDH, respectively. F, Representative western blots of ENAH, E-cadherin, N-cadherin, Snail, Bcl-2, Bcl-xl, Bax, CD133, Nanog and OCT-4 proteins and their quantitation in KYSE70 cells, relative to GAPDH. * p < 0.05 vs. KYSE70 cells co-cultured with exo-NC-mimic; # p < 0.05 vs. KYSE70 cells co-cultured with exo-NC-inhibitor by one-way ANOVA. Data are shown as mean ± standard deviation of three technical replicates