Fig. 7

Delivery of miR-375 by HUCMSC-derived exosomes repressed tumor growth of KYSE70 cells in vivo. The tumor-bearing nude mice were injected with normal saline, KYSE70 cells treated with exo-miR-375 agomir or exo-NC-agomir. A, Tumor volume in nude mice. B, Representative images of tumors in nude mice. C, HE staining of mouse tumor tissues and positive expression of ENAH, Ki-67, E-cadherin and Bcl-2 proteins in tumor tissues from nude mice detected by immunohistochemistry (scale bar = 25 μm). D, miR-375 expression and mRNA expression of ENAH, Bcl-2, Bcl-xl, Bax, CD133, Nanog and OCT-4 was determined by RT-qPCR in tumor tissues, relative to GAPDH or U6, respectively. E, Representative western blots of ENAH, E-cadherin, N-cadherin, Snail, Bcl-2, Bcl-xl, Bax, CD133, Nanog and OCT-4 proteins and their quantitation in tumor tissues, relative to GAPDH. The data are shown as mean ± standard deviation. Data in panel B-E were analyzed using one-way ANOVA and data in panel A were analyzed using repeated measures ANOVA. * p < 0.05 vs. nude mice injected with normal saline. # p < 0.05 vs. nude mice injected with KYSE70 cells treated with exo-NC-agomir. n = 10 for mice. Following each treatment