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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: PDCD6 cooperates with C-Raf to facilitate colorectal cancer progression via Raf/MEK/ERK activation

Fig. 2

PDCD6 inactivation decreases tumor cell growth in vitro and in vivo.a. RT-PCR expression analysis of PDCD6 mRNA in HCT116 and HCT15 cell lines. The data are presented as the means ± SDs, N = 3; and ***, P < 0.0001. b. Immunoblotting of PDCD6 protein expression in HCT116 and HCT15 cell lines. c. Cell proliferation assays. The samples were assayed in triplicate. Each datapoint is the mean value of three independent samples. d. Colony formation assays. The representative images and bar graphs are from three independent experiments. The data are presented as the means ± SDs, N = 3; *P < 0.05; and **, P < 0.01. e. Images of xenograft tumors. The tumors were removed and photographed. N = 6. f. Growth curves of xenograft tumors. Tumor volumes were monitored every 3 days by measuring tumor diameters. The data are presented as the means ± SDs; N = 6. g. Weights of xenograft tumors. The tumors were removed, photographed, and weighed. The bar graphs show the means ± SDs; N = 6; ***, P < 0.001; and ****, P < 0.0001

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