Fig. 3

CPEB3 inhibits EMT induced by TAM-derived IL-6 in CRC cells (a) IL-6 expression was detected in THP-1 macrophages, HCT116, or LoVo, and TAMs with 24 h of co-culture using ELISA; error bars, SEM. (b) IL-6 expression was detected in HCT116 or LoVo and TAMs with or without 24 h of co-culture using qRT-PCR; error bars, SEM. (c) Cell Counting Kit-8 was used to quantify the number of HCT116-Ctrl/CPEB or LoVo-shCtrl/shCPEB3 with or without IL-6 (20 ng/mL); error bars, SEM. (d) A colony formation assay was used to quantify the number of spheres of IL-6 (20 ng/mL)-supplemented HCT116-CPEB3 cells and the control; error bars, SEM. (e) A colony formation assay was used to quantify the number of spheres of IL-6 (20 ng/mL)-supplemented LoVo-shCPEB3 cells and the control; error bars, SEM. (f) The invasion of IL-6 (20 ng/mL)-supplemented HCT116-CPEB3 cells and the control was measured by a Transwell assay (200× magnification); error bars, SEM. (g) The invasion of IL-6 (20 ng/mL)-supplemented LoVo-shCPEB3 cells and the control was measured by a Transwell assay (200× magnification); error bars, SEM. (h) Cell Counting Kit-8 was used to quantify the cell numbers of LoVo/RKO-shCPEB3 cells, cultured TAM supernatants, and IL-6-depleted TAM supernatants. (i) The invasion of LoVo/RKO-shCPEB3 cells, co-cultured TAMs, and IL-6-depleted TAMs co-cultured with LoVo or RKO cells was measured by a Transwell assay (200× magnification); error bars, SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001