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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: CPEB3 inhibits epithelial-mesenchymal transition by disrupting the crosstalk between colorectal cancer cells and tumor-associated macrophages via IL-6R/STAT3 signaling

Fig. 5

CPEB3 modulates CCL2 secretion in CRC cell supernatants to regulate TAM polarization (a) We measured the expression of the respective inflammatory cytokines in cell culture supernatants of HCT116-Ctrl/CPEB3 cells by ProcartaPlex combinable panels; error bars, SEM. (b) We measured the expression of the respective inflammatory cytokines in the supernatants of LoVo-shCtrl/shCPEB3 cells by ProcartaPlex combinable panels; error bars, SEM. (c) THP-1 macrophages were co-cultured with LoVo-shCtrl/shCPEB3 with or without CCL2-neutralizing antibody (1 μg/mL) for 24 h. Flow cytometry was used to explore the surface expression of CD86 and CD163 in the differentiated macrophages; error bars, SEM. (d) THP-1 macrophages were co-cultured with RKO-shCtrl/shCPEB3 cells with or without a CCL2-neutralizing antibody (1 μg/mL) for 24 h. Flow cytometry was used to explore the surface expression of CD86 and CD163 in the differentiated macrophages. Error bars, SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

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