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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: NCSTN promotes hepatocellular carcinoma cell growth and metastasis via β-catenin activation in a Notch1/AKT dependent manner

Fig. 2

NCSTN promotes HCC cell growth and metastasis in vitro. a The effects of NCSTN knockdown and overexpression were examined by western blotting analysis in HCCLM3 and Hep3B cells. Loading control was assessed by β-actin. b CCK8 assays showed NCSTN depletion inhibited cell growth of HCCLM3 and NCSTN overexpression promoted cell growth of Hep3B. c, d Colony formation assays showed colony numbers in HCC cells with NCSTN depletion or overexpression. e, f The cell cycle assays showed that NCSTN depletion increased the G0/G1 fraction and decreased the S and G2/M fraction in HCCLM3 cells, whereas NCSTN overexpression decreased the G0/G1 fraction and increased the S and G2/M fraction in Hep3B cells. g, h The migration and invasion capacity was determined in the indicated HCC cells. Scale bar, 100 μm. i, j Wound healing assays showed the migration capacity of indicated HCC cells. Scale bar, 100 μm. HCC, hepatocellular carcinoma; CCK8, cell counting kit-8. *p < 0.05, **p < 0.01, ***p < 0.001

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