Fig. 4

Enforced expression of circNTRK2 mitigated miR-140-3p-mediated inhibition of ESCC progression in vitro. (a-g) Eca-109 and KYSE-109 cells were transfected with miR-NC, miR-140-3p, or miR-140-3p + circNTRK2. (a) The miR-140-3p expression level was examined by qRT-PCR. (b) The cell viability was determined through CCK-8 assay. (c) The number of EdU-positive cell rate was detected by EdU assay. (d) Colony formation assay was performed to evaluate the colony-forming capability. (e) Transwell assay was used to measure the invasive ability of ESCC cells. (f) Western blot assay was used to measure the protein expression of E-cadherin and vimentin in ESCC cells. (g) Apoptosis analysis was conducted with Annexin V-FITC/PI double staining and flow cytometry. *P < 0.05, **P < 0.01