Fig. 3
FTL promoted glioma cells epithelia-mesenchymal transition in vitro and in vivo. a Distribution of FTL expression in different glioma molecular subtypes based on RNAseq in TCGA and Rembrandt datasets. b Correlation between FTL with Vimentin, twist1, snail1 and ZEB1 in TCGA. Pearson test was used for correlation analysis. c Representative images of morphology of U87 and U251 cells transfected with NC-shRNA or FTL-shRNA. Cells were staining with phalloidin. Scale bars,10 μm. Wound healing (d-e) and transwell assays (f-g) were carried out to detect migration and invasion of glioma cells transfected with NC-shRNA or FTL-shRNA. Wound close percentage was calculated by Image J software (Rawak Software, Inc. Germany).p-values were determined by Student’s t-test. n = three independent experiments. h Western blot was used to detect expression of snail1,viementin and E-cadherin expression after knocking down or overexpression of FTL in U87 and U251 cells. GAPDH was used as loading control. i-j U87 and U251 cells were treated with DMSO or CoCl2(400 mM) for 24 h after transfected with NC-shRNA or FTL-shRNA.Transell assay for invasion detection and (k) western blot for E-cadherin,vimentin,snial1 expression measurement.β-actin was used as loading control. l Fifteen Nude mice were randomly divided into two groups. Images of the xenograft tumors formed in nude mice injected with FTL-shRNA cells and control cells. Tumor volume and tumor weight were calculated (m-n). o Representative images of IHC staining of FTL,snail1 and vimentin. Scale bars,50 μm. All images represented as the mean ± SD of three independent experiments;*,P < 0.5;**,P < 0.01;***, P < 0.001