Fig. 5
FTL enhanced resistance to temozolomide (TMZ) chemotherapy in glioma cells. a CCK-8 assay was used to detect the survival rate of U87 and U251 cells treated with different concentrations of TMZ. b-c Then U87 and U251 cells treated with certain TMZ concentrations(400 μM). The apoptosis cells were detected by flow cytometry and calculated by Fluorescence-activated cell-sorting (FACS). To investigate the role of FTL in mediating hypoxia-promoted EMT.U87 and U251 cells were treated with DMSO or CoCl2(400 mM) for 24 h after transfected with NC-shRNA or FTL-shRNA. Annexin V staining and clone formation were used to detect apoptosis (f-g) and proliferation (d-e) of glioma cells treated with TMZ(400 μM). Six Nude mice were randomly divided into two groups. Images of the xenograft tumors formed in nude mice injected with FTL-shRNA cells and control cells. All mice received two cycles of intraperitoneal injection of TMZ (50 mg/kg/day,5 day/cycle). h-i Representative images of TUNEL staining. Scale bars,20 μm. j Schematic showing hypoxia-induced FTL regulates EMT by regulating AKT/GSK3β/β-catenin signaling. All images represented as the mean ± SD of three independent experiments. *,P < 0.5;**, P < 0.01;***, P < 0.001