Fig. 4

MiR-374a-3p is a target of circTADA2A. a A total of 28 candidate target genes of circTADA2A were simultaneously predicted by circbank and starbase softwares. b The expression of miR-374a-3p in colon carcinoma tissues (n = 450) and normal tissues (n = 8) from starbase v3.0 project was shown as a diagram. c The abundance of miR-374a-3p was examined in CRC tissue samples (n = 70) and normal tissue samples (n = 70) by qRT-PCR. d The expression of miR-374a-3p was determined in CRC cells and NCM460 cells by qRT-PCR. e The correlation analysis was conducted to assess the linear relationship between miR-374a-3p and circTADA2A in CRC tissues. f The predicted binding sequence between miR-374a-3p and circTADA2A and the mutant sites in circTADA2A were marked in red. g and h) Dual-luciferase reporter assay was performed to verify the interaction between miR-374a-3p and circTADA2A in LoVo and HCT116 cells. i and j RIP assay was conducted to test the combination between miR-374a-3p and circTADA2A in CRC cells. *P < 0.05, ***P < 0.001