Fig. 7
From: CircTADA2A suppresses the progression of colorectal cancer via miR-374a-3p/KLF14 axis
KLF14 interference recovers the malignance of CRC cells which is restrained by the transfection of anti-miR-374a-3p. a The level of miR-374a-3p was detected in CRC cells transfected with anti-miR-con or anti-miR-374a-3p by qRT-PCR. b Western blot assay was performed to detect the protein level of KLF14 in LoVo and HCT116 cells transfected with si-con or si-KLF14. c-p LoVo and HCT116 cells were transfected with anti-miR-con, anti-miR-374a-3p, anti-miR-374a-3p + si-con or anti-miR-374a-3p + si-KLF14. c-f Flow cytometry was applied to detect the cell cycle and apoptotic rate of CRC cells. g-j Seahorse XFe 96 Extracellular Flux Analyzer was used to detect the ECAR and OCR of CRC cells. k-p Glucose Uptake Colorimetric Assay kit, Lactate Assay Kit II and ATP Colorimetric Assay kit were utilized to determine the glucose uptake and the production of lactate and ATP in CRC cells. **P < 0.01, ***P < 0.001