Fig. 2

MAPK4 knockout increases the sensitivity of cervical cancer cells to radiation. a Relative protein levels of MAPK4 in SiHa or caSki WT cells and MAPK4 KO cells after radiation treatment, as determined using western blotting. b Colony formation analysis of SiHa or caSki WT cells and MAPK4 KO cells after radiation treatment. The number of cell clones was quantified and shown as histogram. c The 53BP1 and γH2AX levels in SiHa or caSki WT cells and MAPK4 KO cells after radiation treatment, as determined using immunofluorescence. Representative images of three independent experiments are shown (Scale bar, 5 μm). d Relative protein levels of p-DNA-PK and RAD51 in SiHa or caSki WT cells and MAPK4 KO cells after radiation treatment, as determined using western blotting. e Relative RAD51 mRNA levels in SiHa or caSki WT cells and MAPK4 KO cells after radiation treatment, as determined using qRT-PCR. Data are expressed as the mean ± SEM