Fig. 3From: IFI6 depletion inhibits esophageal squamous cell carcinoma progression through reactive oxygen species accumulation via mitochondrial dysfunction and endoplasmic reticulum stressIFI6 promotes cell proliferation, inhibits apoptosis and ameliorates oxidative stress in ESCC. a-b. Representative images (a) and statistical quantification (b) of EdU staining in ESCC cell lines transfected with IFI6-shRNA or ShControl lentivirus. EdU: red, Hoechst 33342: blue. The data are presented as the means and SDs (n = 3). Scale bar: 20 μm. Statistical significance was determined by two-tailed Student’s t-test. ***P < 0.005. c. Representative images (upper) and statistical quantification (lower) of apoptotic and necrotic cell populations in ESCC cell lines, as determined by Annexin-V FITC/PI staining and flow cytometry. Cells with a FITC− and PI− signature were considered viable. Cells with a FITC+ and PI− or a FITC+ and PI+ signature were considered nonviable. The data are presented as the means and SDs (n = 3). Statistical significance was determined by two-tailed Student’s t-test. **P < 0.01. d. Representative images (upper) and statistical quantification (lower) of ROS production assay results in ESCC cells. The indicated cells were stained with carboxy-H2DCFDA and observed under a fluorescence microscope. H2DCFDA: green, Hoechst 33342: blue. Scale bar: 20 μm. The data are presented as the means and SDs (n = 3). Statistical significance was determined by two-tailed Student’s t-test. **P < 0.01. e-f. Representative images (left) and statistical quantification (right) of ROS production assay results in Eca109 (e) and TE-1 (f). The indicated cells were preincubated with different ROS inhibitors, stained with carboxy-H2DCFDA and observed under a fluorescence microscope. H2DCFDA: green, Hoechst: blue. Scale bar: 20 μm. The data are presented as the means and SDs (n = 3). Statistical significance was determined by one-way ANOVA. **P < 0.01Back to article page