Fig. 4

ROS accumulation is responsible for the IFI6 silencing-induced reduction in cell viability. a. Representative images (left) and statistical quantification (right) of EdU staining in the indicated Eca109 cells preincubated with different ROS inhibitors. EdU: red, Hoechst 33342: blue. Scale bar: 20 μm. The data are presented as the means and SDs (n = 3). Statistical significance was determined by one-way ANOVA. ***P < 0.005. b. Representative images (left) and statistical quantification (right) of the apoptosis assay results in Eca109 cells, as indicated by the mitochondrial membrane potential. The indicated cells were stained with JC-1 after preincubation with different ROS inhibitors. Cells stained with JC-1 are visible as either green (J-monomers) or red (J-aggregates) fluorescence. The apoptosis rate was calculated as the ratio of JC-1 aggregates to JC-1 monomers. Scale bar: 20 μm. The data are presented as the means and SDs (n = 3). Statistical significance was determined by one-way ANOVA. ***P < 0.005