Fig. 6

β-catenin signaling regulates cathepsin B expression. a. Quantitative real-time PCR analysis of CTSB expression in Huh-7 cells treated with indicated inhibitors. *** p < 0.0001, one-way ANOVA. b-c. Western blot analysis of cathepsin B in Huh-7 (b) and HepG2 (c) cells treated with increasing amounts of TWS119. d-e. Western blot analysis of indicated proteins in Huh-7 (d) and HepG2 (e) cells transfected with negative control siRNA (NC) or increasing amounts of siGSK-3β. f-g. Western blot analysis of indicated proteins in Huh-7 (f) and HepG2 (g) cells transfected with empty vector or increasing amounts of GSK-3β. h-i. Quantitative analysis of CTSB promoter luciferase reporter activity in Huh-7 cells transfected with siGSK-3β (h) or overexpressing GSK-3β (i). * p < 0.05, ** p < 0.01, Student’s t-test. j-k. Quantitative real-time PCR analysis of CTSB expression in Huh-7 (j) and HepG2 (K) cells transfected with empty vector or β-catenin S33Y. ** p < 0.01, *** p < 0.0001, Student’s t-test. l-m. Western blot analysis of indicated proteins in Huh-7 (l) and HepG2 (m) cells transfected with empty vector or β-catenin S33Y. n. Quantitative analysis of CTSB promoter luciferase reporter activity in Huh-7 cells transfected with empty vector or β-catenin S33Y. * p < 0.05, Student’s t-test