Fig. 7
From: CD147 promotes collective invasion through cathepsin B in hepatocellular carcinoma
CD147 activates β-catenin signaling via downregulating GSK-3β. a-b. Representative images of immunofluorescent staining of CD147, β-catenin and nucleus (DAPI) in Huh-7 (a) and HepG2 (b) cells transfected with negative control siRNA or siCD147. Scale bar, 50 μm. Graph shows quantitative analysis of Pearson’s correlation coefficient between β-catenin and DAPI. * p < 0.05, Student’s t-test. c-d. Western blot analysis of indicated proteins in separated nuclear and cytosolic fragments in Huh-7 (c) and HepG2 (d) cells transfected with negative control siRNA or siCD147. e. The effect on TOP/FOP reporter activity in indicated cells transfected with negative control siRNA or siCD147 was proved by dual-luciferase assay. A Renilla transfection control normalized all results. ** p < 0.01, * p < 0.05, Student’s t-test. f-g. Western blot analysis of indicated proteins in Huh-7 (f) and HepG2 (g) cells transfected with negative control siRNA or siCD147. h-i. Western blot analysis of indicated proteins in Huh-7 (h) and HepG2 (i) cells transfected with empty vector or CD147