Fig. 2

Circ-CPA4 inhibited let-7 miRNA expressions in NSCLC cells by acting as a RNA sponge. a The binding sites of circ-CPA4 and let-7 miRNA were predicted by using the online starBase software (http://starbase.sysu.edu.cn/). Dual-luciferase reporter gene system assay was performed to validate the binding sites of circ-CPA4 and let-7 miRNA in b A549, c H1299, d SK-MES-1 and e Calu-3 cells, respectively. Let-7 miRNA enriched in the pull-down production with the circ-CPA4 probe in f A549, g H1299, h SK-MES-1 and i Calu-3 cells, respectively. Each experiment repeated at least 3 times. *P < 0.05, **P < 0.01, NS means no statistical significance