Fig. 6

Circ-CPA4 regulated NSCLC cell proliferation and death by targeting let-7 miRNA/PD-L1 axis in vitro and in vivo. Knock-down of circ-CPA4 inhibited cell proliferation in a A549 and b H1299 cells by upregulating let-7 miRNA and downregulating PD-L1. c, d The colony formation abilities were inhibited by knocking down circ-CPA4 through let-7 miRNA/PD-L1 axis. e, f Trypan blue staining assay was performed to evaluate cell viability in NSCLC cells. g-i The apoptosis ratio of NSCLC cells were determined by using the FCM assay. Each experiment repeated at least 3 times. *P < 0.05, **P < 0.01, NS means no statistical significance