Fig. 8

NSCLC cells derived PD-L1 inactivated CD8⁺ T cells in the transwell co-culturing system. a The schematic diagram of the transwell co-culturing system. b The cell proliferation abilities of CD8⁺ T cells were inhibited by NSCLC cells derived PD-L1 in the co-culturing system. c Trypan blue staining assay suggested that NSCLC cells derived PD-L1 inhibited CD8⁺ T cell viability. d-f FCM was employed to determine the apoptosis ratio of CD8⁺ T cells in the co-culturing system. g, h Real-Time qPCR was conducted to determine the mRNA levels of IFN-γ, IL-4 and IL-10 in CD8⁺ T cells. i, j The expression levels of IFN-γ, IL-4 and IL-10 in the supernatants of the co-culturing system were determined by using ELISA assay. Each experiment repeated at least 3 times. *P < 0.05, **P < 0.01, NS means no statistical significance