Circular RNA circ-CPA4/ let-7 miRNA/PD-L1 axis regulates cell growth, stemness, drug resistance and immune evasion in non-small cell lung cancer (NSCLC)

Table 5 Primer sequences for Real-Time qPCR

Gene	Primer sequences (strand)
β-actin	Forward: 5′-CTCCATCCTGGCCTCGCTGT-3′
β-actin	Reverse: 5′-GCTGCTACCTTCACCGTTCC-3′
U6	Forward: 5′-GACTATCATATGCTTACCGT-3′
U6	Reverse: 5′-GGGCAGGAAGAGGGCCTAT-3′
Circ-CPA4	Forward: 5′- ACAGCATCTGGTGTGTGCTT-3′
Circ-CPA4	Reverse: 5′-CCCTTTCCTGCAAAACTAGC-3′
Let-7	Forward: 5′-AGCAAGCTTTGGCACCCACCCGTAGAAC-3′
Let-7	Reverse: 5′-TAAGGATCCGATGCAGGGACAAGGACAGAA-3′
PD-L1	Forward: 5′-GCCGAAGTCATCTGGACAAG-3’
PD-L1	Reverse: 5′-TCTCAGTGTGCTGGTCACAT-3’
OCT4	Forward: 5′-AGCGATCAAGCAGCGACTA-3’
OCT4	Reverse: 5′-GGAAAGGGACCGAGGAGTA-3’
SOX2	Forward: 5′-CATCACCCACAGCAAATGAC-3’
SOX2	Reverse: 5′-CAAAGCTCCTACCGTACCACT-3’
Nanog	Forward: 5′-GCAGGCAACTCACTTTATCC −3’
Nanog	Reverse: 5′-CCCACAAATCACAGGCATAG-3’
ALDH1	Forward: 5′-AGCCTTCACAGGATCAACAGA-3’
ALDH1	Reverse: 5′-GTCGGCATCAGCTAACACAA-3’
IFN-γ	Forward: 5′-CCGAAACAGGCTCCCAACCG-3’
IFN-γ	Reverse: 5′-GGGAAGACGCTCTCACCT-3’
IL-4	Forward: 5′-AACGGCTCGACAGGAACCT-3’
IL-4	Reverse: 5′-ACTCTGGTTGGCTTCCTTCCA-3’
IL-10	Forward: 5′-ACAGCCGGGAAGACAATAAC-3’
IL-10	Reverse: 5′- CAGCTGGTCCTTTGTTTGAAA-3’

ISSN: 1756-9966