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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: MicroRNA-148a-3p inhibits progression of hepatocelluar carcimoma by repressing SMAD2 expression in an Ago2 dependent manner

Fig. 2

SMAD2 promotes migration, invasion and proliferation of HCC cells. a Western blot analysis of endogenous SMAD2 expression in HCC cell lines. GAPDH as loading control. b Western blot analysis of SMAD2 knocking down or overexpression efficiency in the indicated cells. GAPDH as loading control. c Representative images of migration and invasion assays after stably knocking down or overexpressing SMAD2 in MHCC-97H or HLF cells (upper panel). Scale bar: red bar, 25 μm. Quantification of cells migrated or invaded (lower panel). d, e CCK8 and EdU assays after stably knocking down or overexpressing SMAD2 in MHCC-97H or HLF cells. Quantification of percentages of Edu+ cells. Data are shown as Mean ± SEM. *, p < 0.05. **, p < 0.01

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