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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: MicroRNA-148a-3p inhibits progression of hepatocelluar carcimoma by repressing SMAD2 expression in an Ago2 dependent manner

Fig. 5

miR-148a inhibits mobility and proliferation of HCC cells in vitro and in vivo. a Representative images of migration and invasion assay in the miR-148a overexpressing MHCC-97H or miR-148a knocking down HLF cells. Scale bar: red bar, 25 μm. Quantification of cells migrated and invaded. b CCK8 assay for the indicated cells. c BALB/c nude mice were subcutaneous injected with the indicated cell. Quantification for tumor volume and weight of subcutaneous tumors in the indicated groups. d Representative images of SMAD2 and Ki67 staining of subcutaneous tumors in the indicated groups. Scale bar: red bar, 25 μm. Quantification of SMAD2 IHC scores and Ki67+ HCC cell percentages. e HCC cells were injected into nude mice by tail veins to establish HCC lung metastasis animal model. Quantification of observed metastatic tumor nodules during autopsy and metastatic niches in H&E stained slides. f Representative images of H&E staining for lung. Scale bar: red bar, 25 μm. Data are shown as Mean ± SEM. *, p < 0.05. **, p < 0.01

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